Tissue Culture — Plant Propagation By

: The explant is moved to a medium enriched with hormones (often cytokinins like BAP) to stimulate the growth of multiple new shoots.

: The fragile plantlets are gradually transitioned from the high-humidity sterile environment to soil and natural light conditions. Essential Requirements Plant Propagation by Tissue Culture

To succeed, tissue culture requires a highly controlled environment: : The explant is moved to a medium