Flow Cytometry In Neoplastic Hematology Morphol... Site

As cells pass a laser beam, the machine reads their size (forward scatter) and internal complexity (side scatter).

Cells are suspended in fluid and tagged with fluorescent antibodies. Flow Cytometry in Neoplastic Hematology Morphol...

Everything begins with a blood smear or bone marrow aspirate. Under the microscope, a pathologist looks for "blasts"—cells that have lost their way. Are the cells abnormally large? The Nucleus: Is the chromatin clumped or fine? The Clues: Presence of Auer rods or specific granules. As cells pass a laser beam, the machine

The true power lies in the overlap. A pathologist might see "monomorphous medium-sized blasts" (Morphology) and use Flow Cytometry to confirm they are actually "CD10+ B-lymphoblasts." As cells pass a laser beam